![]() Our study provides interesting insights into the distribution of the hydrolytic potential of the highly compartmentalised higher termite gut. From an ecological perspective, we could speculate that the capacity to feed on distinct polymorphs of cellulose retained in soil might have enabled this termite species to widely colonise the different habitats of the Amazon basin. We also evidenced that the host cellulases have different phylogenetic origins and structures, which is possibly translated into their different specificities towards cellulose. labralis gut microbes expressed a high diversity of carbohydrate active enzymes involved in cellulose and hemicelluloses degradation, making the soil-feeding termite gut a unique reservoir of lignocellulolytic enzymes with considerable biotechnological potential. Contrary to expectations, we observed that L. We showed that the Labiotermes gut was dominated by members of the Firmicutes phylum, whose abundance gradually decreased towards the posterior segments of the hindgut, in favour of Bacteroidetes, Proteobacteria and Verrucomicrobia. We relied on 16S rRNA gene community profiling, metagenomics and (meta)transcriptomics to uncover the distribution of functional roles, in particular those related to carbohydrate hydrolysis, across different gut compartments and among the members of the bacterial community and the host itself. In this work, we applied an integrative multi-omics approach for the first time at the holobiont level to study the highly compartmentalised gut system of the soil-feeding higher termite Labiotermes labralis. Although soil-feeding termites account for 60% of the known termite species, their biomass degradation strategies are far less known compared to their wood-feeding counterparts. pH, O 2 and H 2 partial pressure), and also differs in the density and structure of residing microbial communities. The digestive tract of soil-feeding higher termites is characterised by radial and axial gradients of physicochemical parameters ( e.g. It includes the host, its gut microbiome and fungal gardens, in the case of fungi-growing higher termites. The termite gut system is often referred to as a micro-reactor and is a complex structure consisting of several components. First-time users of CLC Gx on our workstation computers must complete the Workstation Request Form.Termites are among the most successful insects on Earth and can feed on a broad range of organic matter at various stages of decomposition.All USC users can freely access the software on our workstation computers.Equipped with dual-CPU and 512GB RAM, one of our workstation computers is configured specifically to handle large data set and computationally intensive tasks such as de novo genome assembly and sequencing alignment.Wilson Dental Library, the University Park Campus.Norris Medical Library (RM203A), the Health Sciences Campus.The software has been installed on multiple workstation computers:.On workstation computers in the libraries.Mandatory registration is required for installing CLC Gx on your computer. Please submit the Local Installation Request Form.The computer must be connected to the USC network, either via Ethernet cable on campus or via USC VPN when using wireless (applies to both on- and off-campus wireless connections).Minimum hardware requirement for de novo assembly, metagenomics, and raw reads alignment:: 32GB RAM and Intel i7-6700 or faster processor.Minimum hardware requirement for general use: 16GB RAM and Intel i7-2600 or faster processor.The license consists of TWO concurrent user seats. USC has licensed CLC Gx for the free use of USC faculty, students and staff. ![]()
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